The purpose of this research was to establish a DSPE-RP-HPLC method of simultaneously determining six kinds of trace phthalate plasticizers in food and simulants. The extraction conditions using HLB as sorbent material for enrichment and purification were analyzed by orthogonal experiments. The resolution solvent determined is acetonitrile 6 mL, magnesium135mg, and sodium chloride120 mg. The result showed that the linear regression correlation coefficient for peak area to concentration is above 0. 999; the relative standard deviation is 0. 3% ~4. 7%(n =5); the average recovery is 62. 9% ~105. 0%; and the detection limit is 0. 019 ~0. 046 mg/ kg. It was concluded that this method is simple, efficient, low cost, and higher recovery.