The cushioning properties of honeycomb paperboard under different degrees of vibration fatigue were studied. Paperboard of different vibration fatigue degrees was tested under several humidity environments. Quasi-static compression was carried out and the stress-strain curve, relationship curve of cushioning coefficient and humidity, vibration frequencies, plastic deformation, and energy absorption curve were obtained with Matlab software. The results showed that material bearing capacity, residual yield force, the cushioning effect, and energy absorbing characteristic were weakened with the increasing number of vibration and relative humidity; the strain were different. It was concluded that various properties of honeycomb paperboard are weakened by vibration fatigue. The purpose was to provide theoretical basis for design of cushioning packaging products under different environment and logistics conditions.