Antibacterial paper for packaging was prepared with quaternary ammonium salt of chitosan as antibacterial ingredients, and inhibition zone method was applied to evaluate its antibacterial effect on escherichiacoli and staphylococcus aureus. The effects of different concentration of quaternary ammonium salt of chitosan on thickness, tensile strength, bursting strength, and antibacterial property of the antibacterial paper were studied. The results showed that the strength tensile and bursting strength increase, but the thickness doesn't change significantly; the antibacterial paper can meet the requirement of packaging; the antibacterial effect of the antibacterial paper enhances with the increasing contents of quaternary ammonium salt of chitosan; the antibacterial performance of antibacterial paper is better on escherichiacoli than staphylococcus.