Quadratic general rotary combinational design was applied to optimize the packaging material formulation. The mathematical model was established aimed at the tensile strength of chitosan/ TiO2 antibacterial fresh-keeping blend film based on three factors, namely, the dosages of chitosan, glycerin, and TiO2. The optimal condition was obtained after analysis the mathematical model, where formulation was consisted of chitosan 3. 9 g / 100 mL, glycerin 0. 9 mL/ 100 mL and TiO2 0. 37 g / 100 mL. Under the formulations, the 21. 37 MPa highest tensile strength of the blend film was reached, which was consistent with estimated value of 20. 52 MPa. This result proved that the mathematical model is reliable.