Compound solution was mixed with lemon grass oil and PVA; LDPE film was coated with compound solution and antimicrobial bag was prepared used the coated LDPE film. The volatilization of essential oil in film under high humidity condition and the fresh-keeping effect of grape stored under room temperature were studied. The results showed that the concentration of volatile oil increases with increasing storage time; the bag of 3. 5% lemon grass oil concentration has the best fresh-keeping effect for grape, and its storage period is extended to eighth day and is double of control group.