Fully degradable cellulose film was prepared by NMMO progress. The influence of coagulation bath temperature on mechanical strength, swelling property, antibacterial agent retention rate, and antibacterial properties was studied. The results showed that with the increase of coagulation bath temperature, the tensile strength of the cellulose film and the elongation at break has a certain reduction; the swelling properties in different food simulants change; the change of coagulation bath temperature can cause huge decrease of the retention rate of thymol in the film, so that the inhibitory effect is reduced accordingly.