A FE model of fruit & vegetable preservation packaging box with various designs of ventilation holes were established with respect to stress distribution. The principal design parameters of ventilation holes of fruit & vegetable preservation packaging box were discussed. The result showed that the appropriate pattern with oblong-shaped, close to carton middle, even hole number with symmetrical distribution can minimize stress concentration, which avoids considerably decrease in compression strength of the box. The purpose was to provide reference for structural design of ventilation holes of fruit & vegetable preservation packaging box.