We obtained biomass cellulose micro / nano fiber with uniform dimension using chemical and mechanical methods and prepared film, then observed their microscopic structure and tested performance of the nanofiber films. The results showed that cellulose nanofibers can be obtained after processing, with the diameter between 30 nm and 100 nm, and the nanofiber films exhibits higher strength; the tensile strength of the kraft paper and the corrugated paper are 107. 4 MPa and 121. 8 MPa; their elastic modulus are 5. 6 GPa and 5. 2 GPa respectively. Meanwhile, tensile strength of the nanofiber films is 5 times and 8 times higher than that of the base paper. It also demonstrated that homogeneous and centrifugal treatment can increase performance of the material significantly.