The software structure and function of color overprint deviation vision inspection system and register marks image segmentation algorithm was analyzed according to the characteristics and demands computer vision automatic inspection and color overprint deviation inspection. The software was developed using object-oriented method under VC+ + development environment. Automatic and manual detection modes were designed; the tested data were stored in the database; and report forms were generated and printed. The software solves the color overprint deviation inspection problem and has been used in overprint precision detection, which greatly improves detection efficiency.