Moisture absorption performance of PVA/MC film was studied using by uniform design method. Four factors seven levels uniform design test was set up. The factors were the volume rate of PVA/MC, glycerol dosage, pH, and glutaraldehyde (GA) concentration. The index was moisture absorption rate. The data was analyzed with multivariate linear regression by using SPSS statistical analysis software. The results showed that the significant order of influencing factors on moisture absorption rate of the film is GA concentration > glycerol > volume rate >pH; the optimal formula of the film is PVA /MC rate 2∶1, glycerol 0.11 mL/100 mL, pH 3.06, and GA concentration 2.26×10-4 mol/L; the manufactured film has moisture absorption of 2.68, tensile strength 42.17 MPa, and elongation 48.2%.