The vibration loads on fruit during transportation were simulated using apple as the object. Sweep frequency vibration tests were carried out on single packaging and the natural frequency was measured. Frequency sweep vibration tests were carried out on 10 layers of the package to study the vibration transmission property. The natural frequency, maximal respond acceleration and vibration transmission rate of the bottom, the middle (the sixth layer) and the top layer were determined. The changing rules of maximal respond acceleration and vibration transmission rate with time were discussed. The relation among natural frequency, peak vibration transmission rate, and stack height was analyzed. The damage rule of fruit under simulated vibration condition was studied. The shortage of current package was found and reasonable packaging scheme was put forward.