In order to preserve quality and prolong the shelf life of spinach at room temperature, the preservation effects of modified atmosphere packaging on spinach were studied. The spinach were packaged in four different gas proportions of O-2, CO-2, and N-2, and stored at room temperature. The weight loss, respiration speed, Vc content, chlorophyll content and sense index of spinach were measured each 2 days. The results indicated that the modified atmosphere packaging of 10%O-2+10%CO-2+ 80% N-2 presented better preservation effects than the other groups at room temperature and the spinach's shelf life can reach 8 days under this condition.