In order to research the vibration regulation of package in the circulation environment, based on the 6DOF transport packaging system which was composed of vehicle and package, the vibration model with white noise for road irregular excitation was established. Mathematical model of road irregular excitation, dynamics models of vehicle and package were also established. With the Matlab/Simulink simulation techniques the random vibration frequency domain analysis of transport packaging system was carried out. The magnitude spectrum and power spectrum density of random vibration acceleration of product and fragile parts were obtained. The simulation results showed the relationship between random vibration strength and frequency and fully reflected the random vibration rules. The purpose was to provide theoretical basis for cushion packaging design.