The influencing factors of screen printing process on RFID antenna printing precision were analyzed, which were the mesh count of screen, the characteristics of screen stencil, the angle of doctor blade, and so on. RFID tag antennas were printed through optimization of process parameters and the main parameter, antenna impedance, was measured. The results showed that the fluctuation of antenna impedance is small; screen printing technology can meet the printing quality requirement of RFID tag antenna.