A method for measuring volatile organic compound (VOC) in gravure ink was established by using Gas chromatography (GC) and SPME technology. The influencing factors on extracton efficiency including extraction time, extraction temperature, and film thickness of SPME coating fiber were optimized. The experiment results showed that GC oven temperature program was seting at an initial temperature of 50 ℃, raising to 200 ℃ at 50 ℃/min, then raising to 230 ℃ at 10 ℃/min, and hold for 5 min. An extraction time of 20 min and an extraction temperature of 80 ℃ were the optimized SPME extraction conditions. A 100 μm polydimethyl-siloxane (PDMS) SPME fiber was selected.