The modified bone glue was prepared by grafting copolymerization with glutaraldehyde as modifier, and then it was intercalated by montmorillonite. The influencing factors and performance of glutaraldehyde-bone glue intercalated by montmorillonite was studied and discussed. The performance was characterized. The result showed that the optimum process conditions are reaction temperature 30 ℃, reaction time 1 h, and pH value 7; the toughness and thermal stability of the bone glue is improved under these conditions.