Grafted antibacterial cellulose film (AA-PHMB-g-cellulose film) was used in packaging of fresh pork at room and low temperatures. The indices of total volatile basic N, pH value, aerobic bacterial count and sensory quality, comparing with blank group and common cellulose film packaging group, were tested in normal temperature and refrigeration storage conditions. The experimental results showed that whatever the room temperature or cold storage conditions, the AA-PHMB-g-cellulose packaging film can prolong the fresh pork shelf life effectively.