Aiming at three common Internet environments in China and three popular formats as FLV, WMV, and MP4, objective evaluation method of Quaternion singular value was applied to evaluate digital videos with different format, code rate, resolution that are compressed from the same video, and using subjective experiment to validate its accuracy. The result showed that FLV format gets better evaluation with the good resolution under 512 kb/s; WMV gets lower evaluation than FLV and MP4 under 1 Mb/s and 2 Mb/s; Over 2 Mb/s, video of MP4 format has a lowest distortion, so it has obvious advantage on resolution and visual effect.