The antibacterial kraft paper with silver-loaded zeolite was prepared. The migration of antibacterial agent at 40 ℃ in distilled water, 4%(V/V) acetic acid, 65% ethanol, Olive oil, and N-hexane were studied by using AAS. Results showed that the initial concentration of silver-loaded zeolite in antibacterial kraft paper with the addition of 0.5% antibacterial agent is 2.85 mg/L; while the time of migration is 2 h, 24 h, 96 h, 240 h, and 960 h respectively, there are no antibacterial agent in kraft paper with 0.0% antibacterial agent; however, migration of antibacterial paper with 0.5% silver-loaded zeolite in distilled water, 4% acetic acid, 65% ethanol are detected, and the migration amount are 65% ethanol>4% acetic acid>distilled water; moreover, antibacterial agent is hardly found in Olive oil and N-hexane.