The process feature of screen printing RFID tags antenna was introduced. The influences of antenna resistance from antenna ink layer thickness and the conductivity of the conductive printing ink was put forward based on the analysis of electronic tag antenna resistance calculation method. The influence of screen, doctor blade, and mesh on the ink layer thickness of antenna, and the influence of the viscosity and drying effect of conductive ink on the conduction performance of the antenna were analyzed. The purpose was to provide reference for regulation of the resistance of RFID tags antenna.