In order to prevent the glass from mildewing in storage and transportation, anti-mildew powder should be added between the glasses. The automatic electrostatic glass duster realized online powder spraying; the spraying rate varied depending on the transmission speed of glass. The homogeneity of powder and the adsorption force was improved by electrostatic technology. The control system based on s7-200 PLC was designed; the hardware configuration of the control system, distribution of input-output points and software design were discussed. The test results showed that the dusting amounts are between 0.4~1.6 grams per square meter; about 85% of powder can adsorb on glass; system function and accuracy can meet the needs of safe storage and transportation completely.