The influence of vacuum degree, environmental conditions, and storage time on storage quality of rice was discussed. Commercially available rice was selected as sample. The rice was packaged by vacuum packaging method at various vacuum degrees and stored under different environmental conditions. The fatty acid value, water and reducing sugar content in the sample were determined periodically. The result was analyzed with orthogonal design method and the main factors influencing storage quality of rice were determined. The results showed that the vacuum degree is the most important factor in short time.