Tobacco stem binderless particleboard was prepared by ordinary hot-pressing and steam injection hot-pressing respectively, and mechanical properties of particleboard with different hot-pressing method was studied and analyzed. The results showed that, compared with ordinary hot-pressing, MOR, MOE and IB of the tobacco stem binderless particleboard with steam injection hot-pressing increased significantly; TS decreased significantly. The possible reason was that chemical changes of tobacco stem particles with different Hot-pressing method were different.