In order to research the effect of dots distribution state on color reproduction and color difference, sample strips simulating dots juxtaposition, dots all overprint and dots part overprint were designed. The density, ΔE and spectral curve of print samples were measured and compared. The results showed that, for the same original color reproduction, color performance has obvious difference when original dots distribution style is different; color reproduction is best when dots juxtaposition; color reproduction is the worst when dots completely overlap; the differences of color reproduction also relate to dot area coverage and ink attributes.