Plackett-Burman (PB) design and central composite design (CCD) were combined to optimize 11 technological parameters for preparation of corn stalk fiber cushioning materials. PB design and analysis showed that the three key factors, fiber granularity, fiber content and adhesive content are significant for preparation of corn stalk fiber cushioning material. CCD design and response surface analysis showed that the optimal preparation conditions is fiber granularity 224.62, fiber volume fraction 22.78% and adhesive mass fraction 35%; the strength of the prepared material is 1.758 MPa.