A method of diethylhexyl phthalate quantitative analysis in inner liner of beer bottle by high performance liquid chromatography (HPLC) was established. The results were optimized by sample pre-treatment and the variation of chromatographic detection conditions. The retention time of peak was qualitatively employed to evaluate the results, and the ex-situ measurement was used to analyze quantitatively. When samples were extracted by soaking in ethanol solution, methanol: water = 95∶5 (volume /volume) was as mobile phase, and scanned with SPDM detector at 275 nm, the optimal separation phases was obtained. The results showed that standard curve of DEHP has good linearity with the correlation coefficient r2 higher than 0.998 and the precision was lower than 1.5%. It was confirmed that DEHP immigrates from the inner liner of beer bottle, the concentrations reach to 24.45% in ethanol solution, and 15.97% in 95% methanol solution.