Microcapsule was preparcd with eucalyptus oil embedded beta cyclodextrin. Antibacterial cellulose film was prepared by adding the microcapsule into cellulose solution. Microcapsule embedding ratio, MIC, eucalyptus oil content in the film was determined. The results showed that the maximum embedding ratio is 72. 0% ; the MIC of antibacterial cellulose film is 2. 0% ; the oil quality is l. 16% of the antibacterial cellulose film when the MIC is 2. 0%.