Effects of Oligomeric Proanthocyanidins on Postharvest Preservation and Physiological Changes of Red Raspberries

CHENG Kaili; LU Zirui; YANG Yu; ZHANG Yunhe

doi:10.19554/j.cnki.1001-3563.2026.19.007

PDF(1667 KB)

Packaging Engineering ›› 2026, Vol. 47 ›› Issue (19) : 65-75. DOI: 10.19554/j.cnki.1001-3563.2026.19.007

Agro-products Preservation and Food Packaging

Effects of Oligomeric Proanthocyanidins on Postharvest Preservation and Physiological Changes of Red Raspberries

CHENG Kaili¹, LU Zirui¹, YANG Yu², ZHANG Yunhe^1,*

Author information +

History +

Abstract

The work aims to investigate the effects of exogenous oligomeric proanthocyanidins (OPCs) on the postharvest preservation and physiological changes of red raspberries, providing a theoretical basis for the development of novel natural preservatives. "Heritage" red raspberries were treated with sterile water (control) or different concentrations of OPCs (10, 15, and 20 g/L) and stored at 4 ℃. Fruit quality parameters and physiological indicators associated with antioxidant capacity were evaluated periodically during storage. All OPCs treatments effectively delayed quality deterioration in red raspberries. Compared with the control, the 10, 15, and 20 g/L OPCs treatments better maintained fruit firmness, slowed the decline in titratable acidity (TA) and total soluble solids (TSS), and reduced decay incidence. Among these treatments, 15 g/L OPCs exhibited the best overall preservation performance. On the 16^th day of storage, fruit treated with 15 g/L OPCs maintained a firmness of 0.95 N, with a decay rate of only 22.62% compared with 62.33% in the control. Meanwhile, OPCs treatment reduced malondialdehyde (MDA) accumulation (0.51 μmol/g vs. 1.27 μmol/g in the control), increased ascorbic acid (AsA) content to 1.9-fold that of the control, maintained higher total phenolic content, and significantly enhanced the activities of monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), and phenylalanine ammonia-lyase (PAL). Exogenous application of 15 g/L OPCs enhances both the AsA regeneration cycle and phenylpropanoid metabolism, and these changes are accompanied by improved antioxidant capacity and disease resistance. This treatment significantly reduces the decay rate and delays quality deterioration in red raspberries on the 16^th day of storage, indicating its potential as a green and natural preservative.

Key words

oligomeric proanthocyanidins (OPCs) / red raspberry / postharvest preservation / ascorbic acid / phenylpropanoid metabolism

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

CHENG Kaili, LU Zirui, YANG Yu, ZHANG Yunhe. Effects of Oligomeric Proanthocyanidins on Postharvest Preservation and Physiological Changes of Red Raspberries[J]. Packaging Engineering. 2026, 47(19): 65-75 https://doi.org/10.19554/j.cnki.1001-3563.2026.19.007

References

[1] MOVAHHED HAGHIGHI T, MOUSAVI S S, KOHANSAL Y, et al.Biopolymer-Based Edible Films and Coatings for Berry Preservation: A Systematic Review[J]. Packaging Technology and Science, 2025, 39(2): 181-199.
[2] SHAH H M S, SINGH Z, KAUR J, et al. Trends in Maintaining Postharvest Freshness and Quality of Rubus Berries[J]. Comprehensive Reviews in Food Science and Food Safety, 2023, 22(6): 4600-4643.
[3] GAO S Y, ZHANG Z M, YE K F, et al.Comprehensive Characterization of Rubus idaeus L. Polysaccharides: Extraction, Purification, Structural Diversity, Biological Efficacy, and Structure-Activity Relationships[J]. Journal of Ethnopharmacology, 2026, 355: 120677.
[4] 葛超. 二氧化碳结合木霉抑菌剂对红树莓贮藏品质调控研究[D]. 沈阳: 沈阳农业大学, 2025.
GE C.Study on Regulation of Storage Quality of Red Raspberry by Carbon Dioxide Combined with Trichoderma Fungicide[D]. Shenyang: Shenyang Agricultural University, 2025.
[5] 王一凡. 树莓鲜果气调箱贮藏技术研究[D]. 哈尔滨: 东北农业大学, 2022.
WANG Y F.Study on Modified Atmosphere Box Storage Technology of Fresh Raspberry Fruit[D]. Harbin: Northeast Agricultural University, 2022.
[6] ZHANG H, LI J, CHEN Y.In Situ Ca²⁺-Cross-Linking Effects on Pectin-Tragacanth Films for Fresh Raspberry Preservation[J]. Foods, 2026, 15(6): 1124.
[7] 韩絮舟, 吕静祎, 白琳, 等. 采后氯化钙处理对红树莓保鲜的影响[J]. 食品工业科技, 2020, 41(6): 233-238.
HAN X Z, LYU J Y, BAI L, et al.Effect of Postharvest Calcium Chloride Treatment on Preservation of Red Raspberry[J]. Science and Technology of Food Industry, 2020, 41(6): 233-238.
[8] SHAH H M S, SINGH Z, HASAN M U, et al. Preharvest Methyl Jasmonate Application Delays Cell Wall Degradation and Upregulates Phenolic Metabolism and Antioxidant Activities in Cold Stored Raspberries[J]. Food Chemistry, 2025, 462: 141020.
[9] 杨斯琪. 采后红树莓果实软化机制及褪黑素保鲜研究[D]. 大庆: 黑龙江八一农垦大学, 2024.
YANG S Q.Study on Fruit Softening Mechanism and Melatonin Preservation of Postharvest Red Raspberry[D]. Daqing: Heilongjiang Bayi Agricultural University, 2024.
[10] RAMSÉS G M, PÉREZ R, BERNAL A. Alternatives to Chemical Control of Postharvest Fungal Diseases in Fruits[J]. Horticulturae, 2021, 7(4): 72.
[11] RAHMANZADEH-ISHKEH S, SHIRZAD H, TOFIGHI Z, et al.Efficacy of Nanoemulsion of Satureja sahendica Essential Oil and Melatonin as a Safe Approach Against Postharvest Fungal in Raspberries[J]. Scientific Reports, 2026, 16(1): 38545.
[12] QI Q, CHU M, YU X, et al.Anthocyanins and Proanthocyanidins: Chemical Structures, Food Sources, Bioactivities, and Product Development[J]. Food Reviews International, 2023, 39(7): 4581-4609.
[13] NIE F, LIU L, CUI J, et al.Oligomeric Proanthocyanidins: An Updated Review of Their Natural Sources, Synthesis, and Potentials[J]. Antioxidants, 2023, 12: 1004.
[14] GUO M, LI C Y, HUANG R, et al.Ferulic Acid Enhanced Resistance Against Blue Mold of Malus domestica by Regulating Reactive Oxygen Species and Phenylpropanoid Metabolism[J]. Postharvest Biology and Technology, 2023, 202: 112378.
[15] GUAN Y, LU S, SUN Y, et al.Tea Polyphenols Inhibit the Occurrence of Enzymatic Browning in Fresh-Cut Potatoes by Regulating Phenylpropanoid and ROS Metabolism[J]. Plants, 2024, 13(1): 125.
[16] 刘佳, 陈雨, 赵平, 等. 6′-O-咖啡酰熊果苷处理通过激活苯丙烷代谢提高采后蓝莓果实对灰霉病的抗性[J]. 食品科学, 2024, 45(13): 256-263.
LIU J, CHEN Y, ZHAO P, et al.6′-O-Caffeoylarbutin Treatment Improves the Resistance to Gray Mold of Postharvest Blueberry Fruit by Activating Phenylpropane Metabolism[J]. Food Science, 2024, 45(13): 256-263.
[17] XU W, JIA X, YANG M, et al.Tea Polyphenol Self-Assembly Nanocomposite Coating for Fruit Preservation[J]. ACS Nano, 2025, 19(31): 28146-28159.
[18] RAUF A, IMRAN M, ABU-IZNEID T, et al.Proanthocyanidins: A Comprehensive Review[J]. Biomedicine & Pharmacotherapy, 2019, 116: 108999.
[19] 陈凯丽. 低聚原花青素对苹果采后腐烂病的防治效果和机制研究[D]. 沈阳: 辽宁大学, 2020.
CHEN K L.Study on the Control Effect and Mechanism of Oligomeric Proanthocyanidins on Postharvest Decay of Apple[D]. Shenyang: Liaoning University, 2020.
[20] HUANG G L, LIU T T, MA J J, et al.Anti-Polyphenol Oxidase Mechanism of Oligomeric Procyanidins and Its Application on Browning Control of “Baiyu” Loquat during Storage[J]. Food Bioscience, 2022, 48: 101791.
[21] LUO Z, WU X, XIE Y, et al.Alleviation of Chilling Injury and Browning of Postharvest Bamboo Shoot by Salicylic Acid Treatment[J]. Food Chemistry, 2012, 131(2): 456-461.
[22] RAHMANZADEH-ISHKEH S, SHIRZAD H, TOFIGHI Z, et al.Exogenous melatonin prolongs raspberry postharvest life quality by increasing some antioxidant and enzyme activity and phytochemical contents[J]. Scientific Reports, 2024, 14: 11508.
[23] AKIN M, EYDURAN S P, ÇELIK K, et al.New Approaches in Cold Storage of Fruits: Impact of Postharvest Spermidine and Salicylic Acid Applications on Phenolic Compounds and Quality Characteristics of Raspberry Fruits[J]. Food Science & Nutrition, 2025, 13(6): e70389.
[24] GIDADO M J, GUNNY A A N, GOPINATH S C B, et al. Challenges in Selecting Edible Coating Materials for Fruit Postharvest Preservation and Recent Advances in Edible Coating Techniques: A Review[J]. Journal of Food Science and Technology, 2025, 62: 612-622.
[25] GEST N, GAUTIER H, STEVENS R.Ascorbate as Seen through Plant Evolution: The Rise of a Successful Molecule?[J]. Journal of Experimental Botany, 2013, 64: 33-53.
[26] SMIRNOFF N.Ascorbic Acid Metabolism and Functions: A Comparison of Plants and Mammals[J]. Free Radical Biology and Medicine, 2018, 122: 116-129.
[27] SIEBENEICHLER T J, CRIZEL R L, REISSER P L, et al.Changes in the Abscisic Acid, Phenylpropanoids and Ascorbic Acid Metabolism during Strawberry Fruit Growth and Ripening[J]. Journal of Food Composition and Analysis, 2022, 108: 104398.
[28] SINGH R R, PAJAR J A, AUDENAERT K, et al.Induced Resistance by Ascorbate Oxidation Involves Potentiating of the Phenylpropanoid Pathway and Improved Rice Tolerance to Parasitic Nematodes[J]. Frontiers in Plant Science, 2021, 12: 713870.