Preparation and Properties Evaluation of Chitosan/Polyvinyl alcohol/Menthol Composite Films

WU Yu; JIA Xiuqi; FAN Zhengyang; LYU Zhiyao; CHEN Jiayu; KE Jian; YANG Ke

doi:10.19554/j.cnki.1001-3563.2026.19.002

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Packaging Engineering ›› 2026, Vol. 47 ›› Issue (19) : 13-21. DOI: 10.19554/j.cnki.1001-3563.2026.19.002

Advanced Materials

Preparation and Properties Evaluation of Chitosan/Polyvinyl alcohol/Menthol Composite Films

WU Yu, JIA Xiuqi, FAN Zhengyang, LYU Zhiyao, CHEN Jiayu, KE Jian, YANG Ke^*

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Abstract

The work aims to explore the preparation and properties evaluation of a novel green antibacterial film composed of chitosan (CS)/polyvinyl alcohol (PVA) and menthol, aiming to address traditional plastics' critical challenges, such as lack of antibacterial activity and consequence of environmental pollution. The single-factor variable method was used to optimize the film preparation process by adjusting glycerol concentration, film-forming liquid volume, and drying temperature. Mechanical properties of the composite films were characterized with an electronic universal testing machine, and its antibacterial activity against Escherichia coli and Staphylococcus aureus was assessed through colony count methods. The composite films exhibited superior mechanical properties at 1% (w/w,based on the total mass of the solution) glycerol concentration, 50 mL film-forming liquid volumes, and 45 ℃ drying temperature, and addition of glycerol exerted a significant effect on the mechanical properties. The antibacterial results showed that when menthol concentration reached 8% (w/w), based on the total mass of the solution, the film performed over 95% antibacterial efficacy against both E. coli and S. aureus, revealing broad-spectrum antibacterial characteristics. Therefore, this composite film of both excellent mechanical properties and antibacterial advantages exhibits promising applications in food preservation packaging, providing innovative insights for developing environmentally friendly, efficient antibacterial materials.

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chitosan/polyvinyl alcohol / menthol / antibacterial composite films / mechanical properties / green and environmentally friendly

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WU Yu, JIA Xiuqi, FAN Zhengyang, LYU Zhiyao, CHEN Jiayu, KE Jian, YANG Ke. Preparation and Properties Evaluation of Chitosan/Polyvinyl alcohol/Menthol Composite Films[J]. Packaging Engineering. 2026, 47(19): 13-21 https://doi.org/10.19554/j.cnki.1001-3563.2026.19.002

References

[1] 胡预龙, 张如强, 李文俊, 等. 生物可降解食品包装材料的研究进展[J]. 精细化工, 2025, 42(6): 1211-1220.
HU Y L, ZHANG R Q, LI W J, et al.Research Progress on Biodegradable Food Packaging Materials[J]. Fine Chemicals, 2025, 42(6): 1211-1220.
[2] WANG L Z, SWARUP R, PARYA E, et al.Tannic acid: A Green Crosslinker for Biopolymer-Based Food Packaging Films[J]. Trends in Food Science & Technology, 2023, 136: 11-23.
[3] SHAHID U I, JAISWAL V, BUTOLA B S, et al.Production of PVA-Chitosan Films Using Green Synthesized ZnO NPs Enriched with Dragon Fruit Extract Envisaging Food Packaging Applications[J]. Biol Macromol. 2023, 252: 0141-8130.
[4] RAM K D, LOKESH K, KIRTIRAJ K, et al.Halloysite Nanotubes for Food Packaging Application: A Review[J]. Applied Clay Science, 2023, 234: 106856.
[5] NAGAHAMA H, MAEDA H, KASHIKI T, et al.Preparation and Characterization of Novel Chitosan/Gelatin Membranes Using Chitosan Hydrogel[J]. Carbohydrate Polymers, 2009, 76(2): 255-260.
[6] XIAO Y J, SU B C, YU C Z, et al.Chitosan-Urushiol Nanofiber Membrane with Enhanced Acid Resistance and Broad-Spectrum Antibacterial Activity[J]. Carbohydrate Polymers, 2023, 312:120792.
[7] SEYEDEH M M, MASOUD S, MOJTABA J, et al.Production, Characterization, and Antimicrobial Activity of Almond Gum/Polyvinyl Alcohol/Chitosan Composite Films Containing Thyme Essential Oil Nanoemulsion for Extending the Shelf-Life of Chicken Breast Fillets[J]. International Journal of Biological,2023, 227: 405-415.
[8] 曹亚鹏, 黄雁, 温海晴, 等. 不同配比壳聚糖/聚乙烯醇复合薄膜的制备与性能研究[J]. 塑料包装, 2025, 35(3): 5-8.
CAO Y P, HUANG Y, WEN H Q, et al.Fabrication and Characterization of Ratio-Dependent Chitosan/PVA Composite Films[J]. Plastics Packaging, 2025, 35(3): 5-8.
[9] 张莉琼, 涂志刚, 周秋莹, 等. 聚乙烯醇抑菌包装薄膜研究进展[J]. 塑料包装, 2019, 29(6): 1-6+11.
ZHANG L Q, TU Z G, ZHOU Q Y, et al. Research Progress of Polyvinyl Alcohol Antibacterial Packaging Film[J]. Plastics Packaging, 2019, 29(6): 1-6+11.
[10] 肖铭. 聚乙烯醇与天然高分子材料复合改性研究进展[J]. 精细与专用化学品, 2023, 31(3): 33-36.
XIAO M.Research Progress in Compound Modification of Polyvinyl Alcohol and Natural Polymers[J]. Fine and Specialty Chemicals, 2023, 31(3): 33-36.
[11] 杜珂涵, 张亲心, 黄天姿, 等. 壳聚糖/纳米ZnO复合涂膜对蓝莓保鲜效果的研究[J]. 食品科技, 2023, 48(12): 31-40.
DU K H, ZHANG Q X, HUANG T Z, et al.Effects of Chitosan/Nano-ZnO Composite Coating on Preservation of Blueberry[J]. Food Science and Technology, 2023, 48(12): 31-40.
[12] 巩亚明, 张沓文, 李冬, 等. 壳聚糖/乙酸纤维素/银纳米线复合物的制备及抑菌性能探究[J]. 产业与科技论坛, 2015, 14(24): 39+42.
GONG Y M, ZHANG T W, LI D, et al. Preparation and Antibacterial Properties of Chitosan/Cellulose Acetate/Silver Nanowire Composites[J]. Industrial & Science Tribune, 2015, 14(24): 39+42.
[13] 张群利, 仙鸽, 崔琳琳. 再生纤维素/聚乙烯醇/壳聚糖/二氧化钛抑菌复合膜的制备及性能[J]. 生物质化学工程, 2023, 57(3): 8-14.
ZHANG Q L, XIAN G, CUI L L.Preparation and Performance of Regenerated Cellulose/Polyvinyl Alcohol/Chitosan/TiO2 Antibacterial Composite Film[J]. Biomass Chemical Engineering, 2023, 57(3): 8-14.
[14] 张群利, 马瑞博, 崔琳琳. 聚乙烯醇/淀粉/凤仙透骨草提取物复合膜的制备及性能[J]. 精细化工, 2023, 40(10): 2214-2221.
ZHANG Q L, MA R B, CUI L L.Preparation and Properties of Polyvinyl Alcohol/Starch/Impatiens Balsamina Extract Antibacterial Composite Films[J]. Fine Chemicals, 2023, 40(10): 2214-2221.
[15] 耿直, 付佳乐, 郭婉婷. 葡萄籽提取物复合膜制备及其抑菌性研究[J]. 湖北农业科学, 2023, 62(11): 118-124+130.
GENG Z, FU J L, GUO W T. Research on the Preparation and Antibacterial Activity of Composite Films of Grape Seed Extract[J]. Hubei Agricultural Sciences, 2023, 62(11): 118-124+130.
[16] 黄坤, 黄世豪, 莫璧良, 等. 枇杷叶提取物与壳聚糖复合膜的制备、表征及其在香蕉保鲜中的应用[J]. 食品科技, 2023, 48(11): 198-207.
HUANG K, HUANG S H, MO B L, et al.Preparation and Characterization of Loquat Leaf Extract and Chitosan Composite Film and Its Application in Banana Preservation[J]. Food Science and Technology, 2023, 48(11): 198-207.
[17] 成军, 刘群, 高亚惠, 等. 聚乙烯醇/壳聚糖/芒果皮提取物复合膜的制备与表征[J]. 食品工业科技, 2021, 42(12): 95-102.
CHENG J, LIU Q, GAO Y H, et al.Preparation and Characterization of Polyvinyl Alcohol/Chitosan/Mango Peel Extract Composite Film[J]. Science and Technology of Food Industry, 2021, 42(12): 95-102.
[18] 白静, 李明, 崔丽伟. 聚乙烯醇/马齿苋提取物保鲜膜对猪肉的抑菌保鲜效果研究[J]. 中国塑料, 2023, 37(4): 46-52.
BAI J, LI M, CUI L W.Study on Bacteriostasis and Preservation Effectiveness of Poly(vinyl alcohol)/Portulaca Oleracea Extract Fresh-Keeping Films for Pork[J]. China Plastics, 2023, 37(4): 46-52.
[19] 孙文豪, 杨扬, 陈恒, 等. 薄荷有效成分药理作用研究进展[J]. 江苏中医药, 2023, 55(5): 78-82.
SUN W H, YANG Y, CHEN H, et al.Research Progress on Pharmacological Effects of Active Ingredients in Peppermint[J]. Jiangsu Journal of Traditional Chinese Medicine, 2023, 55(5): 78-82.
[20] 王微, 吴楠, 付玉杰, 等. 薄荷精油抑菌活性研究[J]. 植物研究, 2007, 27(5): 626-629.
WANG W, WU N, FU Y J, et al.Antimicrobial Activities of Mentha Haplocalyx Briq. Essential Oil[J]. Bulletin of Botanical Research, 2007, 27(5): 626-629.
[21] FERREIRA P, CARDOSO T, FERREIRA F, et al. Mentha Piperita Essential Oil Induces Apoptosis in Yeast Associated with Both Cytosolic and Mitochondrial ROS-Mediated Damage[J]. FEMS Yeast Res.2014 Nov; 14(7): 1006-14.
[22] PATEL T, ISHIUJI Y, YOSIPOVITCH G.Menthol: A Refreshing Look at This Ancient Compound[J]. Am Acad Dermatol. 2007, 57(5): 873-8.
[23] JIANG S, LI H,ZhANG L, et al. Generic Diagramming Platform (GDP): A Comprehensive Database of High-Quality Biomedical Graphics. Nucleic Acids Res. 2025; 53(Sup.1): 1670-1676.
[24] 国家标准化管理委员会. 塑料拉伸性能的测定第1部分: GB/T 1040.1-2025[S]. 北京: 中国标准出版社, 2025.
National Standardization Administration of China. Determination of Tensile Properties of Plastics - Part 1: GB/T 1040.1-2025[S]. Beijing: Standards Press of China, 2025.
[25] ZHANG G, GAO N, WANG X, et al.Chitosan/Nanoclusters Membrane-Based Sensors with Antibacterial Properties for Rapid Detection of Bacterial Viability and Food Preservation[J]. Food Hydrocolloids, 2024, 154: 13.
[26] 李文涛. 棉秸秆韧皮纤维-壳聚糖膜复合材料力学性能试验研究[D]. 山西: 山西农业大学, 2024.
LI W T.Mechanical Properties of Cotton Stalk Bast Fiber-Chitosan Membrane Composites Pilot Studies[D]. Shanxi: Shanxi Agricultural University, 2024.
[27] LIM H, HOAG SW.Plasticizer Effects on Physical-Mechanical Properties of Solvent Cast Soluplus® Films[J]. AAPS PharmSciTech, 2013. 14(3): 903-10.
[28] 张雪琴, 罗文翰, 张洁梅, 等. 柔性壳聚糖抑菌膜的制备及性能研究[J]. 包装工程, 2020, 41(15): 119-124.
ZHANG X Q, LUO W H, ZHANG J M, et al.Preparation and Properties of Antibacterial Chitosan-Based Composite Films with Good Flexibility[J]. Packaging Engineering, 2020, 41(15): 119-124.
[29] 赵郁聪, 靳刘萍, 陈满儒, 等. 甘油对壳聚糖-淀粉复合可食性膜力学性能的影响[J]. 包装工程, 2019, 40(21): 68-73.
ZHAO Y C, JIN L P, CHEN M R, et al.Effect of Glycerol on Mechanical Properties of Chitosan-Starch Composite Edible Film[J]. Packaging Engineering, 2019, 40(21): 68-73.
[30] 谭国静, 杨凯, 褚学军. 探究温度对复合膜阻隔性和力学性能的影响[J]. 包装工程, 2023, 44(13): 49-54.
TAN G J, YANG K, CHU Z X.Effect of Temperature on the Barrier and Mechanical Properties of Composite Films[J]. Packaging Engineering, 2023, 44(13): 49-54.
[31] 张营营, 张其林, 周传志. 温度对PTFE膜材料力学性能的影响[J]. 建筑材料学报, 2012, 15(4): 478-483.
ZHANG Y Y, ZHANG Q L, ZHOU C Z.Effects of Temperature on Mechanical Properties of PTFE Coated Fabrics[J]. Journal of Building Materials, 2012, 15(4): 478-483.
[32] 董同力嘎, 王爽爽, 孙文秀, 等. 多层复合聚乳酸薄膜的阻隔性和力学性能[J]. 高分子材料科学与工程, 2015, 31(8): 177-181.
DONG T L G, WANG S S, SUN W X, et al. Barrier and Mechanical Properties of Poly(L-Lactide) Multilayer Films[J]. Polymer Materials Science & Engineering, 2015, 31(8): 177-181.
[33] 李宏彬, 赫光中, 果秋婷. 基于皮尔逊相关系数的有机质谱相似性检索方法[J]. 化学分析计量, 2015, 24(3): 33-37.
LI H B, HE G Z, GUO Q T.Similarity Retrieval Method of Organic Mass Spectrometry Based on the Pearson Correlation Coefficient[J]. Chemical Analysis and Meterage, 2015, 24(3): 33-37.
[34] 周可可, 唐亚丽, 卢立新, 等. 氧化纳米纤维素增强再生纤维素全纤维素复合薄膜的制备及性能[J]. 复合材料学报, 2020, 37(7): 1657-1666.
ZHOU K K, TANG Y L, LU L X, et al.Preparation and Properties of All-Cellulose Composite Films with Oxidized Cellulose Nanofibrils Reinforcing Regenerated Cellulose[J]. Acta Materiae Compositae Sinica, 2020, 37(7): 1657-1666.
[35] 刘铁川, 王闪闪, 桂雅立. 方差分析效果大小报告的新指标[J]. 心理学探新, 2019, 39(3): 238-243.
LIU T C, WANG S S, GUI Y L.A New Effect Size Indicator of Analysis of Variance[J]. Psychological Exploration, 2019, 39(3): 238-243.
[36] 孙廷哲. 以方差分析为例探讨P值决策的局限性[J]. 鲁东大学学报(自然科学版), 2022, 38(2): 152-157.
SUN T Z.Limitation of P-Value Decision Rule: Taking ANOVA as an Example[J]. Journal of Ludong University (Natural Science Edition), 2022, 38(2): 152-157.
[37] SILHAVY TJ, KAHNE D, WALKER S. The Bacterial Cell Envelope[J]. Cold Spring Harb Perspect Biol.2010 May; 2(5): 000414.