Preparation of Anthocyanins of Lycium Ruthenicum Murray Smart Indicator Films and Their Application in Freshness Detection of Chilled Pork

DONG Jing; LIU Yang; WANG Yixuan

doi:10.19554/j.cnki.1001-3563.2026.07.010

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Packaging Engineering ›› 2026, Vol. 47 ›› Issue (7) : 83-91. DOI: 10.19554/j.cnki.1001-3563.2026.07.010

Agro-products Preservation and Food Packaging

Preparation of Anthocyanins of Lycium Ruthenicum Murray Smart Indicator Films and Their Application in Freshness Detection of Chilled Pork

DONG Jing^*, LIU Yang, WANG Yixuan

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Abstract

Lycium ruthenicum Murray are rich in anthocyanins. Changes in acidity and alkalinity can alter the cloud distribution of electrons and conjugated structure of the core part of anthocyanin molecules, thereby affecting their light absorption and causing reversible color changes with changes in environmental pH. The work aims to utilize the natural pH-responsive indicator property of anthocyanins of Lycium ruthenicum Murray to develop a visual freshness indicator film for detecting the freshness of chilled pork. With anthocyanins of Lycium ruthenicum Murray as the indicator, methylcellulose as the matrix, polyethylene glycol-6000 as the toughening agent, and glycerol as the plasticizer, an indicator film was prepared to study the effects of the concentration and amount of anthocyanins of Lycium ruthenicum Murray, glycerol addition, and ethanol concentration on the mechanical properties, moisture content, and water solubility of the film, as well as the pH responsiveness. The experimental results showed that at the anthocyanins of Lycium ruthenicum Murray (LRA) concentration of 25%, ethanol concentration of 45%, and 40 min of ultrasonication, the LRA yield was 14.67 mg/g. The indicator film containing 25% methylcellulose and 3 mL LRA exhibited the best mechanical properties (tensile strength of 25.8 MPa) and moisture barrier performance (moisture content of 8.2%) due to the formation of a dense hydrogen bond network. The LRA indicator film exhibits good pH responsiveness, allowing intuitive monitoring of the freshness of chilled meat, providing a low-cost and efficient solution for quality control in cold chain logistics, and offering a reference for the promotion of edible smart visual monitoring technology in cold chains.

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pH difference method / smart indicator film / anthocyanins of Lycium ruthenicum Murray / freshness detection

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DONG Jing, LIU Yang, WANG Yixuan. Preparation of Anthocyanins of Lycium Ruthenicum Murray Smart Indicator Films and Their Application in Freshness Detection of Chilled Pork[J]. Packaging Engineering. 2026, 47(7): 83-91 https://doi.org/10.19554/j.cnki.1001-3563.2026.07.010

References

[1] 王祎琳, 吕艳娜, 杜健, 等. 食品新鲜度智能指示与传感技术的研究进展[J]. 包装工程, 2025, 46(23): 32-43.
WANG Y L, LYU Y N, DU J, et al.Research Progress in Intelligent Indicating and Sensing Technologies for Food Freshness[J]. Packaging Engineering, 2025, 46(23): 32-43.
[2] 张文雅, 刘香菲, 王烁, 等. 花色苷在食品包装材料中的应用研究进展[J]. 粮食与油脂, 2025, 38(12): 18-23.
ZHANG W Y, LIU X F, WANG S, et al.Research Progress on Application of Anthocyanins in Food Packaging Materials[J]. Cereals & Oils, 2025, 38(12): 18-23.
[3] 刘峣, 李晓雯, 吴鹏军, 等. 食品包装设计研究进展与趋势分析[J]. 包装工程, 2025, 46(22): 268-280.
LIU Y, LI X W, WU P J, et al.Research Progress and Trend Analysis of Food Packaging Design[J]. Packaging Engineering, 2025, 46(22): 268-280.
[4] 赵娅敏, 柴琰, 范荣亮, 等. 基于不同天然指示剂的智能pH响应食品复合膜的研究进展[J] .食品与发酵工业,2026,52(3) : 435-445.
ZHAO Y M, CHAI Y, FAN R L, et al.Progress of Research on Intelligent pH Food Compositefilms Based on Different Natural Indicators[J]. Food and Fermentation Industries, 2026, 52(3): 435-445.
[5] 孙莹, 李沛钊, 刘申,等. 花色苷基智能包装膜在食品保鲜中的应用进展[J]. 食品工业科技, 2025, 46(8): 402-410.
SUN Y, LI P Z, LIU S, et al.Advances in the Application of Anthocyanin-based Smart Packaging Films for Food Preservation[J]. Science and Technology of Food Industry, 2025, 46(8): 402-410.
[6] 原红霞, 侯倩宜, 杜姗, 等. 静乐黑枸杞花青素的纯化及其抗氧化特性[J]. 食品工业科技, 2021, 42(9): 173-178.
YUAN H X, HOU Q Y, DU S, et al.Purification and Antioxidant Properties of Anthocyanins from Jingle Lyciumruthenicum[J]. Science and Technology of Food Industry, 2021, 42(9): 173-178.
[7] 赵娅敏, 张敏, 张沛春, 等. 基于不同提取物中的花青素智能pH复合膜的研究进展[J]. 食品与发酵工业, 2025, 51(1): 373-380.
ZHAO Y M, ZHANG M, ZHANG P C, et al.Advances in Smart pH Complex Membranes Based on Anthocy-Anins from Different Extracts[J]. Food and Fermentation Industries, 2025, 51(1): 373-380.
[8] 中华人民共和国国家卫生健康委员会, 国家市场监督管理总局. 食品安全国家标准预包装食品标签通则: GB 7718—2025[S]. 北京: 中国标准出版社, 2025.
National Health Commission of the People's Republic of China, State Administration for Market Regulation. General Rules for the Label of Prepackaged Foods: GB 7718-2025[S]. Beijing: Standards Press of China, 025.
[9] 崇岚, 潘军辉, 熊鹏文. 智能包装技术的应用现状和发展前景[J]. 包装工程, 2017, 38(15): 149-154.
CHONG L, PAN J H, XIONG P W.Current Status and Development Prospects of Intelligent Packaging Technology[J]. Packaging Engineering, 2017, 38(15): 149-154.
[10] 李田田, 李洋, 王磊明. 智能包装指示器在食品工业中的研究进展[J]. 食品研究与开发, 2019, 40(15): 190-198.
LI T T, LI Y, WANG L M.Research Progress of Intelligent Packaging Indicators in the Food Industry[J]. Food Research and Development, 2019, 40(15): 190-198.
[11] 陈山, 蔺翔宇, 杨瑾, 等. 木香薷花瓣花青素提取工艺优化及其稳定性、抗氧化活性[J]. 食品研究与开发, 2026, 47(2): 141-146.
CHEN S,LIN X Y,YANG J,et al.Extraction Process Optimization and Stability and Antioxidant Activity Evalua‐tion of Anthocyanin from Elsholtzia stauntonii Petals[J]. Food Research and Development, 2026, 47(2): 141-146.
[12] 陈雪, 耿延, 李雯婷, 等. 葡萄皮渣花青素提取工艺研究[J]. 农产品加工, 2024(6): 34-37.
CHEN X, GENG Y, LI W T, et al.Study on Extractiontechnology of Anthocyanin from Grape Skin Residue[J]. Academic Periodical of Farm Products Processing, 2024(6): 34-37.
[13] FU X Z, BELWAL T, CRAVOTTO G, et al.Sono-Physical and Sono-Chemical Effects of Ultrasound: Primary Applications in Extractionand Freezing Operations and Influence on Food Components[J]. Ultra-sonics Sonochemistry, 2020, 60: 104726.
[14] 杨胜龙, 武晓, 罗筑. 聚醚砜/乙烯-丙烯酸复合膜制备及性能[J]. 工程塑料应用, 2024, 52(10): 13-18.
YANG S L, WU X, LUO Z.Preparation and Properties of Polyether Sulfone/Ethylene-Acrylic Acid Composite Membrane[J]. Engineering Plastics Application, 2024, 52(10): 13-18.
[15] GARDI S, GIANNONE L, SARTI G, et al.Influence of Initial Season on PVC Weathering[J]. Polymer Testing, 2023, 125: 108123.
[16] 汤俊炀, 王杨, 江春燕, 等. 基于紫甘蓝花青素的虾新鲜度指示膜的制备及应用[J]. 食品与发酵工业, 2025, 51(24): 43-49.
TANG J Y, WANG Y, JIANG C Y, et al.Preparation and Application for Monitoring Shrimp Freshness of Indicator Films Based on Purple Cabbage Anthocyanin[J]. Food and Fermentation Industries, 2025, 51(24): 43-49.
[17] 李波, 李文, 杨新, 等. 基于紫薯花青素/茜素的猪肉新鲜度智能指示膜研究[J]. 食品与发酵工业, 2024, 50(16): 160-168.
LI B, LI W, YANG X, et al.Development of Intelligent Indicator Films Based on Purple Sweet Potatoanthocyanin/Alizarin for Monitoring Pork Freshness[J]. Food and Fermentation Industries, 2024, 50(16): 160-168.
[18] 国家质量监督检验检疫总局, 中国国家标准化管理委员会. 颜色的表示方法: GB/T 3977—2008[S]. 北京: 中国标准出版社, 2008.
General Administration of Quality Supervision, Inspection and Quarantine of the People’s Republic of China, Standardization Administration of the People’s Republic of China. Specification of Colors: GB/T 3977-2008[S]. Beijing: Standards Press of China, 2008.
[19] FERNANDES E, PINTADO M E, LIMA J L. pH-Sensitive Films Based on κ-carrageenan and Anthocyanins for Food Packaging Applications[J]. Journal of Food Engineering, 2019, 251: 108-115.
[20] SANTOS C A, SILVA R, ROCHA F.Optimization of Anthocyanin Extraction from Grape Pomace Using Response Surface Methodology[J]. Separation and Purification Technology, 2020, 238: 116432.
[21] BINSI P K, FRANCIS G, RAJU M R. pH-Sensitive Films from Polysaccharides and Anthocyanins: A Review[J]. International Journal of Biological Macromolecules, 2020, 164: 3570-3583.
[22] MAJIDI H, SHOJAEE-ALIABADI S, MOOSAVI- MOVAHEDI A A. Color Stability and pH-Sensitivity of Anthocyanin-Based Films: Effect of Environmental Factors[J]. Food Chemistry, 2021, 340: 127789.
[23] 国家标准局. 塑料薄膜拉伸性能试验方法: GB/T 1040.3—2006[S]. 北京: 中国标准出版社, 2006.
National Standards Bureau.Test Method for Tensile Properties of Plastic Films: GB/T 1040.3-2006 [S]. Beijing: China Standards Press, 2006.
[24] 张敏, 李丽, 高艳. 复合膜阻湿性在食品保鲜中的作用[J]. 食品工业科技, 2022, 43(16): 330-336.
ZHANG Min, LI L, GAO Y.The Role of Composite Film Moisture Barrier Properties in Food Preservation[J]. Food Industry Science and Technology, 2022, 43(16): 330-336.
[25] 国家卫生和计划生育委员会. 食品安全国家标准食品接触材料及制品用添加剂使用标准: GB 9685—2016[S]. 北京: 中国标准出版社, 2017.
National Food Safety Standard Standards for the Use of Additives in Food Contact Materials and Products: GB 9685-2016[S] GB 9685-2016[S]. Beijing: Standards Press of China, 2017.