Preparation and Performance Characterization of Edible Alginate/Gelatin Films Loaded with Allicin Microcapsule

ZHANG Xiaocong; WANG Hongjiang; ZHANG Yue; CHEN Kexin; HE Xiaoyue

doi:10.19554/j.cnki.1001-3563.2026.01.002

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Packaging Engineering ›› 2026, Vol. 47 ›› Issue (1) : 10-20. DOI: 10.19554/j.cnki.1001-3563.2026.01.002

Advanced Materials

Preparation and Performance Characterization of Edible Alginate/Gelatin Films Loaded with Allicin Microcapsule

ZHANG Xiaocong¹, WANG Hongjiang^1,2,*, ZHANG Yue¹, CHEN Kexin¹, HE Xiaoyue¹

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Abstract

The work aims to prepare allicin into microcapsules and further add them to films so as to produce water-soluble edible films with antibacterial and antioxidant functions. The microcapsules were prepared according to the process after response surface optimization and were then added to the film-forming matrix composed of sodium alginate and gelatin. The film was cast and then its indicators were measured. The garlicin microcapsule film exhibited excellent mechanical properties, with a tensile strength of (26.33±2.41)MPa and an elongation at break of (21.96±5.26)%. Based on the results of the spectrum and microscopic indicators, it was confirmed that allicin microcapsules existed in the film. The film had sustained-release properties. After being stored at 4 ℃ for 8 days, the allicin remained at a maximum of 46.57% of the initial content. The DPPH free radical scavenging rate of the allicin microcapsule film was up to 43.03%, and the ABTS free radical scavenging rate was up to 54.55%. It inhibited Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. The allicin microcapsule film prepared in this study can be used as a good substitute for traditional petroleum-based plastic films, providing innovative ideas and scientific references for future green, safe, and degradable active packaging.

Key words

allicin / microcapsules / water-soluble film / antibacterial property / antioxidant property

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ZHANG Xiaocong, WANG Hongjiang, ZHANG Yue, CHEN Kexin, HE Xiaoyue. Preparation and Performance Characterization of Edible Alginate/Gelatin Films Loaded with Allicin Microcapsule[J]. Packaging Engineering. 2026, 47(1): 10-20 https://doi.org/10.19554/j.cnki.1001-3563.2026.01.002

References

[1] 谢瑾瑜. 羟丙甲基纤维素/海藻酸钠载药复合材料的制备与性能研究[D]. 上海: 东华大学, 2021.
XIE J Y.Preparation and properties of HPMC/SA drug loading composites[D]. Shanghai: Donghua University, 2021.
[2] 陈婉莹, 高建平, 马晶军. 海藻酸钠复合水凝胶研究进展[J]. 北京服装学院学报(自然科学版), 2022, 42(4): 101-108.
CHEN W Y, GAO J P, MA J J.Research Progress of Sodium Alginate Composite Hydrogel[J]. Journal of Beijing Institute of Fashion Technology (Natural Science Edition), 2022, 42(4): 101-108.
[3] 王浩, 沙小梅, 李鑫, 等. 鱼明胶基可食抗菌膜的制备及性能研究[J]. 食品与发酵工业, 2024, 50(21): 175-182.
WANG H, SHA X M, LI X, et al.Preparation and Performance of Fish Gelatin-Based Edible Antibacterial Film[J]. Food and Fermentation Industries, 2024, 50(21): 175-182.
[4] 张慧玲, 韩艳阳, 刘珊珊, 等. 碱式硫酸锌纳米片作为填料的海藻酸钠薄膜改性[J]. 烟台大学学报(自然科学与工程版), 2025, 38(3): 365-372.
ZHANG H L, HAN Y Y, LIU S S, et al.Modification of Sodium Alginate Films with Zinc Sulfate Hydroxide Nanosheets as Filler[J]. Journal of Yantai University (Natural Science and Engineering Edition), 2025, 38(3): 365-372.
[5] ZHANG F R, SHEN R, LI N, et al.Nanocellulose: An Amazing Nanomaterial with Diverse Applications in Food Science[J]. Carbohydrate Polymers, 2023, 304: 120497.
[6] 于晓慧, 庞一帆, 王梦阳, 等. 壳聚糖-海藻纳米纤维素协同强化海藻酸钠薄膜的制备及其在南美白对虾冷藏保鲜中的应用[J]. 食品工业科技, 2025, 46(14): 243-255.
YU X H, PANG Y F, WANG M Y, et al.Development of Chitosan-Seaweed Nanocellulose Enhanced Alginate Packaging Film for the Refrigerated Preservation of the Litopenaeus Vannamei[J]. Science and Technology of Food Industry, 2025, 46(14): 243-255.
[7] 周士禹. 明胶基复合水凝胶挤出式3D打印成型性能研究[D]. 杭州: 浙江农林大学, 2023.
ZHOU S Y.Gelatin-based Composite Hydrogel Extrusion 3D Printing Performance Study[D]. Hangzhou: Zhejiang A & F University, 2023.
[8] YAN J, HE S C, CHEN L L, et al.Effect of Gelatin-Chitosan-Cyclocarya Paliurus Flavonoids Edible Coating Film on the Preservation of Chilled Beef[J]. LWT, 2024, 199: 116138.
[9] 裴莹, 徐高强, 李玉辉, 等. 纳米纤维素晶须/明胶复合膜的制备与结构及性能[J]. 高分子材料科学与工程, 2017, 33(8): 45-49.
PEI Y, XU G Q, LI Y H, et al.Fabrication, Structure and Properties of Cellulose Nanowhiskers/Gelatin Composite Film[J]. Polymer Materials Science & Engineering, 2017, 33(8): 45-49.
[10] 梁梦茜, 张群利. 蛋白质复合抗菌膜的研究进展[J]. 精细化工, 2025, 42(10): 2089-2097.
LIANG M X, ZHANG Q L.Research Progress on Protein Composite Antibacterial Membranes[J]. Fine Chemicals, 2025, 42(10): 2089-2097.
[11] 裴思阳, 刘照军, 黄姝, 等. 植物精油强化海藻酸钠-明胶可食性复合膜的制备及其抑菌性能研究[J]. 食品安全质量检测学报, 2025, 16(21): 200-209.
PEI S Y, LIU Z J, HUANG S, et al.Preparation and Antibacterial Properties of Plant Essential Oil-Enhanced Sodium Alginate-Gelatin Edible Composite Films[J]. Journal of Food Safety & Quality, 2025, 16(21): 200-209.
[12] PIRAK T, JANGCHUD A, JANTAWAT P.Characterisation of Physical, Chemical and Antimicrobial Properties of Allicin-Chitosan Complexes[J]. International Journal of Food Science and Technology, 2012, 47(7): 1339-1347.
[13] DHWANI S, POOJITHA P, GURUMOORTHI P.A Review on Different Extraction and Quantification Methods of Allicin From Garlic[J]. Journal of Xidian University, 2021, 15(6): 183-196.
[14] 张钰杰. 大蒜精油脂质体的制备及物化特性和抑菌活性[D]. 天津: 天津科技大学, 2022.
ZHANG Y J.Preparation, Physicochemical Propertles and Antibacterial Activity of Garlic Essential Oil Liposomes[D]. Tianjin: Tianjin University of Science & Technology, 2022.
[15] BALASUBRAMANI P, VISWANATHAN R, VAIRAMANI M.Response Surface Optimisation of Process Variables for Microencapsulation of Garlic (Allium Sativum L.) Oleoresin by Spray Drying[J]. Biosystems Engineering, 2013, 114(3): 205-213.
[16] EMADZADEH B, GHORANI B, NAJI-TABASI S, et al.Fate of Β-Cyclodextrin-Sugar Beet Pectin Microcapsules Containing Garlic Essential Oil in an Acidic Food Beverage[J]. Food Bioscience, 2021, 42: 101029.
[17] WANG Y F, SHAO J J, WANG Z L, et al.Study of Allicin Microcapsules in Β-Cyclodextrin and Porous Starch Mixture[J]. Food Research International, 2012, 49(2): 641-647.
[18] 张孝聪, 王洪江, 张悦, 等. 负载大蒜素海藻酸钠/明胶可食性膜在蟹棒保鲜中的应用[J]. 包装工程, 2025, 46(21): 115-123.
ZHANG X C, WANG H J, ZHANG Y, et al.Preparation and Performance Characterization of Edible Alginate/Gelatin Membrane Loaded with Allicin[J]. Packaging Engineering, 2025, 46(21): 115-123.
[19] 国家市场监督管理总局, 国家标准化管理委员会. 食品安全国家标准多肽抗氧化性测定 :DPPH和ABTS法: GB/T 39100—2020[S]. 北京: 中国标准出版社, 2020.
National Market Supervision and Administration Bureau, National Standardization Administration Committee. Food Safety National Standard-Determination of Antioxidant Activity of Peptides: DPPH and ABTS Methods: GB/T 39100-2020[S]. Beijing: China Standards Press, 2020.
[20] ZHENG J Y, HU Y Y, SU C Y, et al.Structural, Physicochemical and Biodegradable Properties of Composite Plastics Prepared with Polyvinyl Alcohol (PVA), OSA Potato Starch and Gliadin[J]. Journal of Food Engineering, 2023, 339: 111278.
[21] 张兵兵, 刘贵珊, 谢德浪, 等. 沙棘油微胶囊复合膜的制备及其对手抓羊肉的保鲜效果[J]. 食品工业科技, 2025, 46(4): 393-403.
ZHANG B B, LIU G S, XIE D L, et al.Preparation of Sea-Buckthorn Oil Microcapsule Composite Film and the Preservation Effect of Hand-Grabbed Mutton[J]. Science and Technology of Food Industry, 2025, 46(4): 393-403.
[22] 于洋. 明胶/海藻酸钠/单宁酸复合膜的制备及其保鲜水果效果研究[D]. 长沙: 湖南大学, 2023.
YU Y.Research on the Preparation of Gelatin/Alginic Acid/Tannic Acid Composite Membrane and Its Effect on Fresh Fruit Preservation[D]. Changsha: Hunan University, 2023.
[23] 张炳. PBAT/PLA复合薄膜阻水改性研究[D]. 泰安: 山东农业大学, 2025.
ZHANG B.Research on Water Resistance Modification of PBAT/PLA Composite Films[D]. Taian: Shandong Agricultural University, 2025.
[24] 刘子聪, 张群利. 鱼皮明胶/羧甲基壳聚糖/橘子精油抑菌复合膜的制备及性能[J/OL]. 精细化工, 2025: 1-12(2025-04-08). https://link.cnki.net/doi/10.13550/j.jxhg.20250017.
LIU Z C, ZHANG Q L. Preparation and Properties of Fish Skin Gelatin/Carboxymethyl Chitosan/Orange Essential Oil Antibacterial Composite Films[J/OL]. Fine Chemicals, 2025: 1-12(2025-04-08). https://link.cnki.net/doi/10.13550/j.jxhg.20250017.
[25] 董小刚, 樊敏仪, 杨静贤, 等. 香芹酚纳米乳液-海藻酸钠复合膜的制备及对冰鲜三文鱼的保鲜效果[J]. 食品科技, 2025, 50(2): 139-146.
DONG X G, FAN M Y, YANG J X, et al.Preparation of Carvacrol Nanoemulsion-Sodium Alginate Composite Film and Its Preservation Effects on Chilled Salmon[J]. Food Science and Technology, 2025, 50(2): 139-146.
[26] JIANG G Y, HOU X Y, ZENG X D, et al.Preparation and Characterization of Indicator Films from Carboxymethyl-Cellulose/Starch and Purple Sweet Potato (Ipomoea Batatas (L.) Lam) Anthocyanins for Monitoring Fish Freshness[J]. International Journal of Biological Macromolecules, 2020, 143: 359-372.
[27] 章斌, 侯小桢, 卢杨学, 等. 具有抗菌和氨敏感功能的海藻酸钠基智能活性薄膜监测虾新鲜度的研究[J/OL]. 食品工业科技, 2025: 1-17(2025-06-23). https://link.cnki.net/doi/10.13386/j.issn1002-0306.2025020161.
ZHANG B, HOU X Z, LU Y X, et al. Research on Sodium Alginate-Based Intelligent-Active Film with Antibacterial and Ammonia-Sensitive Functions for Shrimp Freshness Monitoring[J/OL]. Science and Technology of Food Industry, 2025: 1-17(2025-06-23). https://link.cnki.net/doi/10.13386/j.issn1002-0306.2025020161.
[28] 朱喜成. 缓释防雾抗菌果蔬保鲜膜的制备与性能研究[D]. 无锡: 江南大学, 2024.
ZHU X C.Preparation and Performance Study of Slow-Release Anti-Fog and Antibacterial Fruit and Vegetable Packaging Film[D]. Wuxi: Jiangnan University, 2024.
[29] PENG X L, YANG J P, CUI P L, et al.Influence of Allicin on Quality and Volatile Compounds of Fresh-Cut Stem Lettuce during Cold Storage[J]. LWT-Food Science and Technology, 2015, 60(1): 300-307.
[30] LAN W Q, SHAO Z, DU J T, et al.Insight into the Antibacterial Activity and Mechanism of Allicin and Antioxidant of Bamboo Leaves Against Shewanella Putrefaciens[J]. International Journal of Food Science and Technology, 2023, 59(1): 390-398.
[31] HUANG L R, JIA S F, WU R K, et al.The Structure, Antioxidant and Antibacterial Properties of Thiol-Modified Soy Protein Isolate Induced by Allicin[J]. Food Chemistry, 2022, 396: 133713.
[32] CHEN Y, QIU H Y, DONG M H, et al.Preparation of Hydroxylated Lecithin Complexed Iodine/Carboxymethyl Chitosan/Sodium Alginate Composite Membrane by Microwave Drying and Its Applications in Infected Burn Wound Treatment[J]. Carbohydrate Polymers, 2019, 206: 435-445.
[33] 张雪丽. 大蒜精油β-环糊精包合物脂质体的制备及其降血脂效果评价[D]. 泰安: 山东农业大学, 2024.
ZHANG X L.Preparation of Liposomes Encapsulating Garlic Essential Oil β-Cyclodextrin Complex and Evaluation of Their Lipid-Lowering Effects[D]. Taian: Shandong Agricultural University, 2024.
[34] CHEN C, LIU C H, CAI J, et al.Broad-Spectrum Antimicrobial Activity, Chemical Composition and Mechanism of Action of Garlic (Allium sativum) Extracts[J]. Food Control, 2018, 86: 117-125.
[35] ROSS Z M, O'GARA E A, HILL D J, et al. Antimicrobial Properties of Garlic Oil Against Human Enteric Bacteria: Evaluation of Methodologies and Comparisons with Garlic Oil Sulfides and Garlic Powder[J]. Applied and Environmental Microbiology, 2001, 67(1): 475-480.
[36] JIA X W, LI X, ZHAO J H, et al.Fabrication and Characterization of Crosslinked Pea Protein Isolated/Pullulan/ Allicin Electrospun Nanofiber Films as Potential Active Packaging Material[J]. Food Packaging and Shelf Life, 2022, 33: 100873.