Optimization of PHBV Synthesis Process by Halophilic Bacteria with Rubber Wood Hydrolysate and Valeric Acid Co-matrix

YIN Fen; ZHANG Yutian; LI Dongna; MA Xiaojun; JI Fanyi; HUA Qing

doi:10.19554/j.cnki.1001-3563.2025.21.008

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Packaging Engineering ›› 2025, Vol. 46 ›› Issue (21) : 70-77. DOI: 10.19554/j.cnki.1001-3563.2025.21.008

Advanced Materials

Optimization of PHBV Synthesis Process by Halophilic Bacteria with Rubber Wood Hydrolysate and Valeric Acid Co-matrix

YIN Fen¹, ZHANG Yutian², LI Dongna², MA Xiaojun^2*, JI Fanyi¹, HUA Qing¹

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Abstract

Polyhydroxyalkanoate is a promising alternative to traditional packaging materials as a biopolymer. In order to reduce its cost, the work aims to study the optimization process of synthesizing 3-hydroxybutanoic acid-3-hydroxyvaleric acid copolyester (PHBV) by halophilic bacteria with rubber wood hydrolysate and valeric acid as a co-matrix. The rubber wood hydrolysate was prepared and the PHBV was synthesized with rubber wood hydrolysate and valeric acid as carbon sources for Halomonas venusta DSM4743. The residual rates of total reducing sugars and valeric acid in the culture medium, as well as the intracellular contents of PHBV, 3-hydroxybutyrate (3HB), and 3-hydroxyvalerate (3HV) in halophilic bacteria were measured regularly, to explore the effects of nitrogen source types, NaCl concentration, and pH value on the synthesis process of PHBV. When ammonium chloride was used as the nitrogen source, sodium chloride concentration was 60 g/L, and pH value was 7, the yield of PHBV synthesized by halophilic bacteria was the highest. At 120 hours, the PHBV yield reached 2.01 g/L, with HV monomer accounting for 21.45% and PHBV being 73.05% of the dry weight of the bacterial cells. Under these optimized conditions, the production cost of synthesizing PHBV has been reduced and the yield of PHBV has been increased, presenting good application value.

Key words

poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) / halophilic bacteria / co-fermentation / rubber wood hydrolysate / valeric acid

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YIN Fen, ZHANG Yutian, LI Dongna, MA Xiaojun, JI Fanyi, HUA Qing. Optimization of PHBV Synthesis Process by Halophilic Bacteria with Rubber Wood Hydrolysate and Valeric Acid Co-matrix[J]. Packaging Engineering. 2025, 46(21): 70-77 https://doi.org/10.19554/j.cnki.1001-3563.2025.21.008

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