Biosynthesis of Biodegradable PHA Packaging Materials from Volatile Fatty Acids by Halomonas Venusta

ZHENG Yingying; MA Xiaojun; LI Jianing; YIN Fen; LI Dongna

doi:10.19554/j.cnki.1001-3563.2025.21.004

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Packaging Engineering ›› 2025, Vol. 46 ›› Issue (21) : 31-40. DOI: 10.19554/j.cnki.1001-3563.2025.21.004

Advanced Materials

Biosynthesis of Biodegradable PHA Packaging Materials from Volatile Fatty Acids by Halomonas Venusta

ZHENG Yingying¹, MA Xiaojun^1*, LI Jianing², YIN Fen³, LI Dongna¹

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Abstract

The work aims to develop a Halomonas venusta strain with exceptional VFAs tolerance and systematically elucidate its VFAs metabolic mechanisms, to address the critical issue of limited volatile fatty acid (VFAs) tolerance (typically<5 g/L) in halophilic bacteria that restricts polyhydroxyalkanoate (PHA) accumulation efficiency, so as to improve the production efficiency of PHAs. First, by establishing concentration gradients of different VFAs, the tolerance of the strain to various VFAs was examined. Second, by comparing PHA production, PHA yield, and PHA productivity when different VFAs were used as carbon sources, the optimal carbon source type for the strain was identified. Finally, by analyzing parameters such as carbon source utilization rate and consumption rate during the fermentation cycle, the metabolic patterns of the strain's dependence on VFAs were revealed. Results demonstrated significant differential VFAs tolerance in Halomonas venusta: butyrate (50 g/L) > acetate/valerate (15 g/L) > propionate. Optimal production conditions yielded 1.81 g/L PHB (73.98 wt%) with 30 g/L butyrate and 1.01 g/L PHBV (59.41 wt%) with 15 g/L valerate. Metabolic analysis revealed characteristic biphasic consumption patterns and significant negative correlations between both biomass yield (Y_x/s) and product yield (Y_p/s) with carbon source concentration. In conclusion, compared with other halophilic strains, Halomonas venusta exhibits overall superior tolerance to VFAs, effectively improving PHA production efficiency. This study provides a theoretical basis and data support for optimizing the PHA production process.

Key words

polyhydroxyalkanoates / Halomonas venusta / production efficiency / volatile fatty acids

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ZHENG Yingying, MA Xiaojun, LI Jianing, YIN Fen, LI Dongna. Biosynthesis of Biodegradable PHA Packaging Materials from Volatile Fatty Acids by Halomonas Venusta[J]. Packaging Engineering. 2025, 46(21): 31-40 https://doi.org/10.19554/j.cnki.1001-3563.2025.21.004

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