The work aims to explore the effect of microporous controlled atmosphere packaging combined with preservative on the preservation of cut rose flowers at low temperature. The cut rose 'Corolla' was treated with 20-hole controlled atmosphere packaging (M20), 20-hole controlled atmosphere packaging combined with 1-methylcyclopropylene (1-MCP) (M20+1-MCP) and 20-hole controlled atmosphere packaging combined with 1-MCP and SO2 plastic preservation paper (M20+1-MCP+SO2). Fresh cut flowers were stored at (4±0.3) ℃ and their sensory, respiratory intensity, ethylene production rate, soluble protein content, total phenol content and antioxidant enzyme activity were measured regularly. Compared with M20, M20+1-MCP and M20+1-MCP+SO2 treatment group could better maintain the sensory quality of cut flowers during storage. The biochemical components of anthocyanin, soluble protein, total phenol and total flavone inhibited respiration and ethylene production, and slowed down the accumulation of malondialdehyde (MDA), a marker of oxidative damage and maintained the REDOX balance of reactive oxygen species (ROS). After 30 days of storage, the activities of PAL, chitinase and β-1, 3-glucanase in M20+1-MCP+SO2 treatment group were 68.87 U/g, 18.64 U/g and 14.87 U/g, respectively, which were significantly higher than those in other treatment groups and peroxidase (POD) and superoxide dismutase (SOD) activities were maintained. Bottle life was negatively correlated with MDA content, ethylene production rate and PPO activity (P<0.01), while POD activity was positively correlated with disease resistance and stress resistance. The combination of 1-MCP and SO2 preservative paper with microporous controlled atmosphere packaging plays a synergistic role in preservation through maintaining biochemical components, inhibiting respiration, maintaining REDOX balance and improving disease resistance.