The work aims to study the synergic effect of copper oxide (CuO) and potassium permanganate (KMnO4) on ethylene removal to prepare an ethylene removal agent for fruit and vegetable preservation. With synthetic zeolite as the carrier, aynthetic zeolite/CuO material was prepared by ion exchange and high temperature baking, loaded with KMnO4, and an orthogonal test was designed to optimize the loading process, and the aynthetic zeolite /CuO/KMnO4 ethylene scrubber was prepared. The material was characterized by scanning electron microscope and X-ray diffractometer, and the ethylene removal performance was tested by gas chromatograph. CuO modification and KMnO4 loading did not damage the crystal structure of aynthetic zeolite. The ethylene removal rate was improved after CuO modification with aynthetic zeolite. Under the initial ethylene content of 4.5 mL, the ethylene removal amount of aynthetic zeolite/CuO/KMnO4 ethylene remover was 4.426 mL, and the ethylene removal rate reached 98.4%, which was 37.5% higher than that of aynthetic zeolite/KMnO4 (3.218 mL). CuO and KMnO4 have synergistic effects on ethylene removal, and the aynthetic zeolite/CuO/KMnO4 ethylene removal agent has good ethylene removal effect, which provides support for its application in fruit and vegetable preservation packaging.