To achieve high-performance vibration isolation for different loads simultaneously in the transportation system, the work aims to propose a novel high-performance quasi-zero-stiffness transportation vibration isolation system suitable for various loads by using multiple sets of negative stiffness structures of cams and spring-rollers. Firstly, a quasi-zero-stiffness transportation vibration isolation system adaptable to multiple different loads was designed by using the parallel connection of multiple sets of cams and spring-rollers of negative stiffness mechanisms and positive stiffness springs. Secondly, the static characteristics of the system were analyzed, and its stiffness characteristics were derived. Finally, based on the harmonic balance method and the motion differential equation and phase diagram of the system, the dynamic vibration isolation characteristics of the vibration isolation system under different horizontal spring pre-compression amounts and different excitation amplitudes were studied. By designing appropriate structural parameters of the system, the vibration isolation system would exhibit quasi-zero-stiffness characteristics near multiple different equilibrium points, thereby achieving excellent broadband vibration isolation effects. The new quasi-zero-stiffness transportation vibration isolation system can simultaneously possess quasi-zero-stiffness characteristics under different load conditions. Increasing the horizontal spring pre-compression amount to approach the compression amount that satisfies the quasi-zero-stiffness condition and reducing the excitation amplitude can both improve the vibration isolation performance of the system. Compared with the light load situation, the initial vibration isolation frequency of the quasi-zero-stiffness transportation vibration isolation system under heavy load is lower, and the frequency band range where the frequency jump phenomenon occurs is smaller.