The work aims to reduce the influence of environmental factors such as temperature and relative humidity on the production process of eco-friendly paper bags, achieve precise quantitative gluing, and provide theoretical support for the control system of the eco-friendly paper bag production line; and optimize the relevant process parameters by analyzing the relationship between temperature, relative humidity, glue application thickness, paper pressing time and the peel strength of paper bags. The response surface methodology (RSM) was used to establish a regression model of the peel strength of paper bags with respect to temperature, glue application thickness, relative humidity and paper pressing time. The accuracy of the model was evaluated through variance analysis, fitting accuracy analysis, residual analysis and comparison of predicted values with actual values. The influence of each factor on the peel strength of paper and the strength of their interaction were analyzed through three-dimensional response surface and contour plots. The numerical function in Design Expert software was used to obtain the optimal process parameters, and the reliability of the model was verified through experiments. The factors affecting the peel strength of paper in order were paper pressing time, temperature, glue application thickness and humidity. The interaction between temperature and paper pressing time, and between glue application thickness and paper pressing time had a significant impact on the peel strength. The optimized process parameters were:temperature 15.7 ℃, glue application thickness 12.45 μm, relative humidity 51.12%, and paper pressing time 3.38 s. Through multiple experiments, the error between the predicted values and the actual values is all within 5%, which proves that the regression model has high accuracy and reliability. This study provides a reliable theoretical basis for the optimization of the gluing process in the production of eco-friendly paper bags and has important practical application value.