The work aims to establish a method for simultaneously and rapidly determining four antibacterial and antifungal agents in disposable bamboo and wood chopsticks by gas chromatography-mass spectrometry.The samples were extracted by ultrasonic methanol after crushing or smashing, and dissolved by n-hexane after rotating evaporation. The test solution was separated by DB-5MS quartz capillary column, detected by mass spectrometer, and quantified by external standard method. The Result showed that the established quantitative methods of the four target substances had a good linear relationship (R2>0.99) in the range of 0.2-10 mg/L. Under the three concentrations, the recovery rates were 90.0%-103%, the relative standard deviations were 0.94%-12.6%. The limits of quantification were 0.1 mg/kg for biphenyl and o-phenylphenol, 0.2 mg/kg for apromazole and 0.3 mg/kg for thiamendazole. In conclusion, the method is simple, efficient, sensitive, and accurate, and can be used for rapid screening and determination of antibacterial preservatives in disposable bamboo and wood chopsticks used for food.