It is well known that screen printing electronic technology has been widely used in the fields of biomedical engineering, sensors, and electronic circuits. Investigating the effects of scraper angle, off-grid spacing, and blade pressure on the electrical conductivity of printed circuits is crucial for enhancing the accuracy and quality of printed electronic products in industrial production. Orthogonal experiments were conducted to investigate the effects of blade angle, off-grid distance, and blade pressure using screen printing equipment and testing instruments to determine the optimal process conditions for screen printing. Subsequently, these optimal conditions were validated through multi-parameter comparative experiments. The optimal solution obtained through orthogonal experiments was either c2a1b2 or c2b2a1. Results from multi-parameter comparative experiments indicated that the minimum line resistance value corresponded to an off-grid spacing of 1.0 mm or 1.4 mm, a blade angle of 60°, and an equivalent distance for blade pressure of 1.1 mm. Additionally, when the equivalent distance for blade pressure was set at 1.0 cm and the off-grid spacing at 0.8 mm, the fluctuation range of resistance values at different positions along the lines was minimized. The experimental results indicate that the most significant factor affecting the conductivity of printed circuit is the off-grid distance, followed by blade pressure and finally blade angle. The experimental results validate the reliability of orthogonal experimental design in studying the conductive performance of printed circuits. However, there are some shortcomings in its application to the investigation of resistance stability.