The work aims to explore migration behavior of flavoring substances in cigarettes. Seven groups of menthol heated tobacco samples were designed and prepared, and the menthol migration behavior was investigated by regularly analyzing the menthol relative mass of each segment in the cigarette. Results showed that the menthol relative mass of each segment presented different range-ability with the extension of storage time under three storage conditions. Compared with the other two conditions, the relative mass range-ability under refrigeration conditions was smaller, which indicated that refrigeration was more conducive to sample storage. Under the same storage condition, the application amount of essence in each segment of cigarettes affected the change trend and range of its relative mass. With the increase of the essence application amount in this segment, the relative mass showed a decrease trend of great range, otherwise it showed an increase trend of great range. Results of dynamic fitting manifested that the menthol migration behavior of each segment conformed to a pseudo first order kinetic equation, which could better describe the menthol migration process in the cigarette. The study provides ideas for exploring migration behavior of flavoring substances in cigarettes, which can be used for the design and storage of flavored cigarette products.