The work aims to develop a new environmentally friendly fresh film formula to extend the storage and supply time of fruits and vegetables, thus ensuring the nutritional demand of marine navigation personnel for special fruits and vegetables. By adjusting the ratio of starch/biodegradable resin/plasticizer, the thermoplastic starch/polyolefin (TPS/PE) films were prepared, and various environmentally friendly plastic film formulas were developed, including dissolved films and biodegradable films. Five common vegetables subject to four film formulas were tested for preservation effect. The preservation performance of the films was evaluated mainly by monitoring the water loss rate and yellowing rate of the vegetables. The decay rate (water loss rate plus yellowing rate) of vegetables in plastic film was significantly lower than that by traditional preservation method. The average preservation period of the five perishable vegetables was up to 55 d, and the decay rate did not exceed 15%. To sum up, the four kinds of preservation film have a good effect on fruit and vegetable preservation.