The work aims to meet the need for new active packaging materials. In this study, a blended electrospinning process was used to prepare PLGA-HK composite nanofiber membranes to protect food from oxidation and microorganisms. Through FT-IR, scanning electron microscopy, TG, water contact angle and other characterization methods, it was proved that the PLGA-HK composite nanofiber membrane had good morphology, thermal stability and surface hydrophobicity. In addition, the PLGA-HK composite nanofiber membrane showed ≥99% antibacterial activity and (61.33±7.71)% antioxidant activity. Furthermore, it was safe because the active ingredient would not dissolve during the action. In food preservation applications, PLGA-HK composite nanofiber membrane can effectively maintain food quality and extend shelf life. It has the potential to be used as an active packaging material for food preservation applications.