The work aims to investigate the changes in storage quality of "Shine Muscat" grapes treated with 1-MCP combined with low-temperature treatment. Grapes were used as test materials, and treated separately with 0.5, 1, 1.5, 2 μL/L 1-MCP. Those without 1-MCP treatment were used as controls (CK). The test materials were stored in a fresh-keeping warehouse at (0±0.5)℃ and 75% relative humidity, and the physiological indicators of grapes were measured every 20 days. As a result, compared with other concentration treatments, the combination of 1 μL/L 1-MCP and low-temperature treatment suppressed the increase in hardness, respiratory intensity, MDA content, and PPO activity of "Shine Muscat" grapes, reduced the occurrence of grain drop and decay rates, and delayed the decrease in SSC and TA content. In conclusion, the combination of 1 μL/L1-MCP and low-temperature treatment delays the post ripening and aging process of "Shine Muscat" grapes during storage, maximally maintaining the fresh quality of the fruits and prolonging their storage period. It can be used as a practical technology for large-scale storage, transportation, and preservation of "Shine Muscat" grapes.