Considering the low-temperature climate in cold regions and the insulation requirements of most temperature-sensitive products at 2-8 ℃, the work aims to study the insulation performance and influencing factors of environmentally friendly honeycomb cartons to provide a reasonable anti-freeze packaging solution. Firstly, the finite element modeling of the antifreeze insulation box, the experimental testing of the surface emissivity and thermal conductivity of different materials such as honeycomb cardboard, and the analysis of the influence law of the thickness of honeycomb cardboard, pore size and other factors on its thermal conductivity were conducted. Finally, the effect of thickness and the inner attached materials on the thermal insulation performance of honeycomb cartons was analyzed by simulation and experimental comparison. The surface emissivity of honeycomb cardboard was 0.81, and the thermal conductivity decreased slightly with the increase of thickness and aperture of honeycomb cardboard. Through the comparison of simulation and experiment, that of the honeycomb cardboard with bubble films inside was improved by 9% compared with those without inside material, while that of the honeycomb cardboard with reflective foils inside was improved by only 1%. Honeycomb cartons can replace traditional foam materials in cold chain logistics, providing an alternative packaging solution for the sustainable development of cold chain logistics.