The work aims to establish a detection method of photoinitiator in ink to control the safety of food packaging from the source. The ink samples were extracted by ultrasonic with ethyl acetate and acetonitrile and subject to constant volume by the acetonitrile solution with a volume fraction of 20%. After extracted by solid phase extraction column, the samples were subject to constant volume with acetonitrile and determined by HPLC. The 11 components had good linear relationship within their respective ranges (R≥0.999 5), the average recovery rate of sample addition was 90.0%~95.0%, and the relative standard deviation (n=6) was less than 3.5%. A method is established, which can be used for the simultaneous determination of 2-methyl-1-(4-methylthiophenyl)-2-(4-morpholinyl)-1-acetone, 1-hydroxycyclohexylphenyl ketone, 2-benzoylbenzoate methyl ester, 4-dimethylaminobenzoate ethyl ester, 4,4'-bis (N, N-dimethylamino) benzophenone, benzoin dimethyl ether, 4-methylbenzophenone, 4,4'-bis (N,N-diethylamino) benzophenone, isooctyl p-dimethylaminobenzoate, 2-isopropylthioxanthone and 2,4-diethylthiazolidone in the ink.