The work aims to modify corn starch (CS) to prepare of the corn crosslinking oxidized starch adhesive (CCOSA), so as to improve the water resistance and the adhesive strength of CCOSA. CS, biomass materials glutamic acid and hydrogen peroxide were chosen as the main raw materials, crosslinking agent, and oxidant, respectively. Through crosslinking and then oxidizing processes, the CCOSA was prepared. The optimal conditions of the corn crosslinking starch (CCS) and the corn crosslinking oxidized starch (CCOS) were determined by single factor tests, and the structural characteristics, adhesive strength and water resistance were measured. The optimal process conditions of the CCS were as follows:CS was 20.0 g, glutamic acid was 1.0 g, pH value was 9.0, reaction temperature was 55 ℃, and reaction time was 1.5 h. Under this condition, the sedimentation of the prepared CCS was the smallest, while the degree of CCS was the largest. Furthermore, the optimal process conditions of the CCOS were as follows:20 g of CCS, 0.1% the mass of total CCS amount of FeSO4, 2 mL of 30% mass fraction hydrogen peroxide, reaction temperature of 50 ℃, pH value of 3 and reaction duration of 3 h. The carboxyl content of the corn crosslinking oxidized starch reached 0.184% by this process, which has the best oxidation effect on corn crosslinking starch. Fourier infrared spectrum analysis showed that the C—N stretching vibration absorption peaks appeared in the CCS molecule at 1 157.22 cm−1, the C=O stretching vibration absorption peaks appeared in the CCOS molecule at 1 731.35 cm−1, and these absorption peaks moved to a higher wave number, and the intensity of these peaks were significantly higher than that of typical starch derivatives C=O absorption peaks at 1 653.25 cm−1. Under these conditions, the CCOSA were prepared successfully. The adhesive strength of CCOSA was 6.15 MPa. Compared with CCSA, COSA and CSA, its adhesive strength was increased by 38.83%, 107.07%, and 352.21%, respectively. In addition, the water resistance of the CCOSA was improved significantly. The FT-TR analysis shows that CCOSA are prepared successfully through crosslinking and then oxidizing processes, and the water resistance of CCOSA is significantly improved.