The work aims to solve the problem of uneven roll-to-roll printing, and improve the winding accuracy. The principle of winding and guiding of printing press was analyzed. The mathematical model of winding and guiding systems was established. A correction algorithm based on fractional-order sliding mode control was proposed to analyze its stability. Simulation was carried out under the condition of setting different winding line speeds and different misalignment interference input signals. An experiment platform of winding and guiding was established to conduct winding and guiding experiments on the two control methods. The simulation results showed that fractional-order sliding mode controllers had better dynamic performance and steady-state performance than traditional PID controllers. The experimental results showed that the fractional-order sliding mode control algorithm had higher guiding accuracy than traditional PID controllers. In conclusion, the fractional-order sliding mode control algorithm can effectively improve the winding and guiding accuracy of the printing press and meet the requirements of high-precision printing.