The work aims to study the respiration rates of cherry tomatoes at different temperature and characterize the rates with model parameters. Under aerobic conditions, a closed system was adopted and the time-dependent gas concentrations of cherry tomatoes in the closed system were determined over the temperature range of 0-30 °C to calculate the O2 consumption rate and CO2 generation rate. The respiration process of cherry tomatoes was mathematically correlated with enzymatic Michaelis-Menten model. The best packaging material of cherry tomatoes was selected by respiration rate model at 25 °C. The results indicated that the respiration rate of cherry tomatoes was consistent with the Michaelis-Menten model. The determination coefficient R2 was greater than 0.9, and the regression was good. The temperature dependence of respiratory dynamics of cherry tomatoes could be characterized by Arrhenius equation and the determination coefficient was R2>0.99. At the same time, the Michaelis-Menten model was used to verify PE1 and PE2, and it was concluded that PE1 bag was more suitable for packaging cherry tomato. Michaelis-Menten model is important for cherry tomato preservation.