The work aims to prepare dual-active antibacterial agent (DAA) with halogenated amine and quaternary ammonium in mild experiment conditions to investigate its antibacterial activities and its application in fiber-based packaging materials. The standard curve was calibrated with an UV-visible spectrophotometer to investigate the antibacterial activities as well as regeneration and reuse performance of DAA and FAPM carrying the DAA. The results indicated that the sterilization rates of DAA (0.25 g/mL aqueous solution) and FAPM to Escherichia coli and Staphylococcus aureus were all 100% within 15 minutes. After regeneration for 3 times, the sterilization of DAA and FAPM could still reach 100%. In conclusion, DAA has good sterilization effect on Gram-negative bacteria (Escherichia coli) and Galanz-positive bacteria (Staphylococcus aureus). The fiber-based materials carrying DAA have good sterilization effect, and their antibacterial properties can be regenerated many times.