The work aims to solve the problems such as the small specific surface area of g-C3N4 and the fast electron-hole recombination rate leading to poor photocatalytic performance, S-doped graphite phase carbon nitdrive (g-C3N4) was synthesized by pyrolysis polymerization assisted by water vapor activation with urea and thiourea as precursor materials, and photocatalytic antibacterial packaging paper was prepared by interfacial polymerization. The morphology, surface functional groups, physical and photocatalytic antibacterial properties of packaging paper were investigated in detail by SEM, FTIR, WCA, TGA and photocatalytic antibacterial test. The dense g-C3N4 layer effectively improved the hydrophobicity and thermal stability of antibacterial packaging paper. Under visible light irradiation, photocatalytic antibacterial packaging paper could inactivate about 100% of Escherichia coli (E.coli) and Staphylococcus aureus (S.aureus) cells. The base paper without visible light irradiation had poor antibacterial properties compared with the photocatalytic packaging paper. g-C3N4 photocatalytic antibacterial packaging paper has good broad-spectrum antibacterial properties, which provides a new method for the preparation of green antibacterial packaging materials.